Evolution of Pap Stain

Pap stain is a universal stain used for gynecologic and non-gynecologic cytology smear. It is mainly used for oral and cervical cancer screening in asymptomatic population and in the follow up of patients with cancer. Pap test has decreased incidence of cervical cancer by 70% in developed countries. Pap stain yields polychromatic transparent staining reaction with crisp nuclear and cytoplasmic features (Asthana A, 2014; RoyBiswas et al., 2008).